Compound Dissolution
Compound Dissolution, Solubilization, and Thawing
Primary dissolution of compounds, thawing, and solubilization of precipitated compounds is a use of AFA that is gaining wide acceptance in the compound management community.
Current dissolution technologies are either inadequate or have significant drawbacks compared to AFA. This is illustrated in the table below:
Feature |
Benefit |
Technique |
AFA |
Vortex |
Sonication |
| Isothermal |
|
Yes |
Yes |
No |
| Non-contact |
- Decapping not necessary
- Washing of instrument not necessary
- No instrument or compound cross contamination
|
Yes |
Yes |
No |
Controllable Mechanical
Energy |
- Fast & highly efficient
- Capable of gum / glue / "glass" dissolution
- Fast
|
Yes |
No |
No/Yes |
| Automation friendly |
|
Yes |
No |
No |
| No consumable |
|
Yes |
Yes |
No |
| Capable of thawing individual wells (3s for a 2D tube) |
- Reduced freeze thaw cycle for plates
|
Yes |
No |
No |
| Plate / tube independent |
|
Yes |
No |
No |
 To date a large amount of data has been generated on the application of AFA in compound dissolution. We have included a video to illustrate this; however data is also presented below in comparison to a microplate Sonicator. The data was analyzed using quantitative liquid chromatography.
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